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mouse embryonic fibroblast nih 3t3 cells  (ATCC)
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ATCC mouse embryonic fibroblast nih 3t3 cells
Characterization and gene editing efficiency of PBAE-Plasmid NPs. ( a ) and ( b ) RNA silencing effects of different sgRNAs targeting JAK1 in <t>NIH-3T3</t> and DC 2.4 cells. ( c ) Size and zeta potential of the PBAE-plasmid complex at various mass ratios. ( d ) Agarose gel electrophoresis of the PBAE/plasmid complex at different mass ratios. ( e ) Size distribution analyzed by dynamic light scattering (DLS) and transmission electron microscopy (TEM) images of PBAE-plasmid NPs at a mass ratio of 20:1. ( f ) and ( g ) Effects of the PBAE-plasmid complex at various mass ratios on NIH-3T3 and DC 2.4 cell viability. ( h ) Green fluorescence in NIH-3T3 cells transfected with PBAE-plasmid NPs. ( i ) JAK1 mRNA expression in NIH-3T3 cells transfected with PBAE-plasmid NPs. ( j ) JAK1 protein expression in mice transfected with PBAE-plasmid NPs. ( k ) Quantitative analysis of (j). Data are presented as mean ± SD (n = 3). Bars sharing the same letter are not significantly different, whereas those with different letters are statistically significant (p < 0.05).
Mouse Embryonic Fibroblast Nih 3t3 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse embryonic fibroblast nih 3t3 cells - by Bioz Stars, 2026-03
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mouse embryonic fibroblast mef cell line  (ATCC)
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ATCC mouse embryonic fibroblast mef cell line
Characterization and gene editing efficiency of PBAE-Plasmid NPs. ( a ) and ( b ) RNA silencing effects of different sgRNAs targeting JAK1 in <t>NIH-3T3</t> and DC 2.4 cells. ( c ) Size and zeta potential of the PBAE-plasmid complex at various mass ratios. ( d ) Agarose gel electrophoresis of the PBAE/plasmid complex at different mass ratios. ( e ) Size distribution analyzed by dynamic light scattering (DLS) and transmission electron microscopy (TEM) images of PBAE-plasmid NPs at a mass ratio of 20:1. ( f ) and ( g ) Effects of the PBAE-plasmid complex at various mass ratios on NIH-3T3 and DC 2.4 cell viability. ( h ) Green fluorescence in NIH-3T3 cells transfected with PBAE-plasmid NPs. ( i ) JAK1 mRNA expression in NIH-3T3 cells transfected with PBAE-plasmid NPs. ( j ) JAK1 protein expression in mice transfected with PBAE-plasmid NPs. ( k ) Quantitative analysis of (j). Data are presented as mean ± SD (n = 3). Bars sharing the same letter are not significantly different, whereas those with different letters are statistically significant (p < 0.05).
Mouse Embryonic Fibroblast Mef Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse embryonic fibroblast mef cell line/product/ATCC
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mouse embryonic fibroblast mef cell line - by Bioz Stars, 2026-03
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mouse embryonic fibroblast nih 3t3 cell line  (ATCC)
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ATCC mouse embryonic fibroblast nih 3t3 cell line
Characterization and gene editing efficiency of PBAE-Plasmid NPs. ( a ) and ( b ) RNA silencing effects of different sgRNAs targeting JAK1 in <t>NIH-3T3</t> and DC 2.4 cells. ( c ) Size and zeta potential of the PBAE-plasmid complex at various mass ratios. ( d ) Agarose gel electrophoresis of the PBAE/plasmid complex at different mass ratios. ( e ) Size distribution analyzed by dynamic light scattering (DLS) and transmission electron microscopy (TEM) images of PBAE-plasmid NPs at a mass ratio of 20:1. ( f ) and ( g ) Effects of the PBAE-plasmid complex at various mass ratios on NIH-3T3 and DC 2.4 cell viability. ( h ) Green fluorescence in NIH-3T3 cells transfected with PBAE-plasmid NPs. ( i ) JAK1 mRNA expression in NIH-3T3 cells transfected with PBAE-plasmid NPs. ( j ) JAK1 protein expression in mice transfected with PBAE-plasmid NPs. ( k ) Quantitative analysis of (j). Data are presented as mean ± SD (n = 3). Bars sharing the same letter are not significantly different, whereas those with different letters are statistically significant (p < 0.05).
Mouse Embryonic Fibroblast Nih 3t3 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse embryonic fibroblast nih 3t3 cell line/product/ATCC
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mouse embryonic fibroblast nih 3t3 cell line - by Bioz Stars, 2026-03
99/100 stars
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mouse embryonic fibroblasts  (ATCC)
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ATCC mouse embryonic fibroblasts
Effects of Bulbophyllum drymoglossum aqueous extract (BDAE) on cell viability, proliferation, and chemical composition. (A) Representative images of Bulbophyllum drymoglossum leaves and aqueous extract (BDAE) preparation by high-temperature extraction (110°C, 15 min). (B) Relative viability of NIH/3T3 <t>fibroblasts</t> and porcine alveolar macrophages (3D4/31) after 24 h exposure to increasing concentrations of BDAE, measured using the WST-8 assay. ns, not significant; **** p<0.0001 versus vehicle control. (C) Effects of BDAE on cell proliferation in 3D4/31 cells during 3 days of culture with indicated extract concentrations. (D) Representative GC–MS total ion chromatogram of BDAE, showing major metabolite peaks. (E) Table of major compounds in BDAE as identified by GC-MS, including retention time, area percentage, compound names, similarity index (SI), and molecular formula. GC-MS, gas chromatography–mass spectrometry.
Mouse Embryonic Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse embryonic fibroblasts/product/ATCC
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mouse embryonic fibroblasts - by Bioz Stars, 2026-03
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mouse embryonic fibroblast cell line nih 3t3  (ATCC)
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ATCC mouse embryonic fibroblast cell line nih 3t3
Effects of Bulbophyllum drymoglossum aqueous extract (BDAE) on cell viability, proliferation, and chemical composition. (A) Representative images of Bulbophyllum drymoglossum leaves and aqueous extract (BDAE) preparation by high-temperature extraction (110°C, 15 min). (B) Relative viability of NIH/3T3 <t>fibroblasts</t> and porcine alveolar macrophages (3D4/31) after 24 h exposure to increasing concentrations of BDAE, measured using the WST-8 assay. ns, not significant; **** p<0.0001 versus vehicle control. (C) Effects of BDAE on cell proliferation in 3D4/31 cells during 3 days of culture with indicated extract concentrations. (D) Representative GC–MS total ion chromatogram of BDAE, showing major metabolite peaks. (E) Table of major compounds in BDAE as identified by GC-MS, including retention time, area percentage, compound names, similarity index (SI), and molecular formula. GC-MS, gas chromatography–mass spectrometry.
Mouse Embryonic Fibroblast Cell Line Nih 3t3, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse embryonic fibroblast cell line nih 3t3/product/ATCC
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mouse embryonic fibroblast cell line nih 3t3 - by Bioz Stars, 2026-03
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healthy cell lines 3t3  (ATCC)
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ATCC healthy cell lines 3t3
Effects of Bulbophyllum drymoglossum aqueous extract (BDAE) on cell viability, proliferation, and chemical composition. (A) Representative images of Bulbophyllum drymoglossum leaves and aqueous extract (BDAE) preparation by high-temperature extraction (110°C, 15 min). (B) Relative viability of NIH/3T3 <t>fibroblasts</t> and porcine alveolar macrophages (3D4/31) after 24 h exposure to increasing concentrations of BDAE, measured using the WST-8 assay. ns, not significant; **** p<0.0001 versus vehicle control. (C) Effects of BDAE on cell proliferation in 3D4/31 cells during 3 days of culture with indicated extract concentrations. (D) Representative GC–MS total ion chromatogram of BDAE, showing major metabolite peaks. (E) Table of major compounds in BDAE as identified by GC-MS, including retention time, area percentage, compound names, similarity index (SI), and molecular formula. GC-MS, gas chromatography–mass spectrometry.
Healthy Cell Lines 3t3, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/healthy cell lines 3t3/product/ATCC
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healthy cell lines 3t3 - by Bioz Stars, 2026-03
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mouse embryonic fibroblast mef cells  (ATCC)
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ATCC mouse embryonic fibroblast mef cells
Effects of Bulbophyllum drymoglossum aqueous extract (BDAE) on cell viability, proliferation, and chemical composition. (A) Representative images of Bulbophyllum drymoglossum leaves and aqueous extract (BDAE) preparation by high-temperature extraction (110°C, 15 min). (B) Relative viability of NIH/3T3 <t>fibroblasts</t> and porcine alveolar macrophages (3D4/31) after 24 h exposure to increasing concentrations of BDAE, measured using the WST-8 assay. ns, not significant; **** p<0.0001 versus vehicle control. (C) Effects of BDAE on cell proliferation in 3D4/31 cells during 3 days of culture with indicated extract concentrations. (D) Representative GC–MS total ion chromatogram of BDAE, showing major metabolite peaks. (E) Table of major compounds in BDAE as identified by GC-MS, including retention time, area percentage, compound names, similarity index (SI), and molecular formula. GC-MS, gas chromatography–mass spectrometry.
Mouse Embryonic Fibroblast Mef Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse embryonic fibroblast mef cells/product/ATCC
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mouse embryonic fibroblast mef cells - by Bioz Stars, 2026-03
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murine control mouse embryonic fibroblasts  (ATCC)
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ATCC murine control mouse embryonic fibroblasts
Effects of Bulbophyllum drymoglossum aqueous extract (BDAE) on cell viability, proliferation, and chemical composition. (A) Representative images of Bulbophyllum drymoglossum leaves and aqueous extract (BDAE) preparation by high-temperature extraction (110°C, 15 min). (B) Relative viability of NIH/3T3 <t>fibroblasts</t> and porcine alveolar macrophages (3D4/31) after 24 h exposure to increasing concentrations of BDAE, measured using the WST-8 assay. ns, not significant; **** p<0.0001 versus vehicle control. (C) Effects of BDAE on cell proliferation in 3D4/31 cells during 3 days of culture with indicated extract concentrations. (D) Representative GC–MS total ion chromatogram of BDAE, showing major metabolite peaks. (E) Table of major compounds in BDAE as identified by GC-MS, including retention time, area percentage, compound names, similarity index (SI), and molecular formula. GC-MS, gas chromatography–mass spectrometry.
Murine Control Mouse Embryonic Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/murine control mouse embryonic fibroblasts/product/ATCC
Average 99 stars, based on 1 article reviews
murine control mouse embryonic fibroblasts - by Bioz Stars, 2026-03
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Characterization and gene editing efficiency of PBAE-Plasmid NPs. ( a ) and ( b ) RNA silencing effects of different sgRNAs targeting JAK1 in NIH-3T3 and DC 2.4 cells. ( c ) Size and zeta potential of the PBAE-plasmid complex at various mass ratios. ( d ) Agarose gel electrophoresis of the PBAE/plasmid complex at different mass ratios. ( e ) Size distribution analyzed by dynamic light scattering (DLS) and transmission electron microscopy (TEM) images of PBAE-plasmid NPs at a mass ratio of 20:1. ( f ) and ( g ) Effects of the PBAE-plasmid complex at various mass ratios on NIH-3T3 and DC 2.4 cell viability. ( h ) Green fluorescence in NIH-3T3 cells transfected with PBAE-plasmid NPs. ( i ) JAK1 mRNA expression in NIH-3T3 cells transfected with PBAE-plasmid NPs. ( j ) JAK1 protein expression in mice transfected with PBAE-plasmid NPs. ( k ) Quantitative analysis of (j). Data are presented as mean ± SD (n = 3). Bars sharing the same letter are not significantly different, whereas those with different letters are statistically significant (p < 0.05).

Journal: Materials Today Bio

Article Title: Feasibility of combining JAK1 gene editing via CRISPR-CasRx with EGCG–lactoferrin nanoparticle therapy in a microneedle-based platform for atopic dermatitis

doi: 10.1016/j.mtbio.2026.102884

Figure Lengend Snippet: Characterization and gene editing efficiency of PBAE-Plasmid NPs. ( a ) and ( b ) RNA silencing effects of different sgRNAs targeting JAK1 in NIH-3T3 and DC 2.4 cells. ( c ) Size and zeta potential of the PBAE-plasmid complex at various mass ratios. ( d ) Agarose gel electrophoresis of the PBAE/plasmid complex at different mass ratios. ( e ) Size distribution analyzed by dynamic light scattering (DLS) and transmission electron microscopy (TEM) images of PBAE-plasmid NPs at a mass ratio of 20:1. ( f ) and ( g ) Effects of the PBAE-plasmid complex at various mass ratios on NIH-3T3 and DC 2.4 cell viability. ( h ) Green fluorescence in NIH-3T3 cells transfected with PBAE-plasmid NPs. ( i ) JAK1 mRNA expression in NIH-3T3 cells transfected with PBAE-plasmid NPs. ( j ) JAK1 protein expression in mice transfected with PBAE-plasmid NPs. ( k ) Quantitative analysis of (j). Data are presented as mean ± SD (n = 3). Bars sharing the same letter are not significantly different, whereas those with different letters are statistically significant (p < 0.05).

Article Snippet: Mouse embryonic fibroblast NIH/3T3 cells and mouse dendritic DC2.4 cells were obtained from the American Type Culture Collection (ATCC).

Techniques: Plasmid Preparation, Zeta Potential Analyzer, Agarose Gel Electrophoresis, Transmission Assay, Electron Microscopy, Fluorescence, Transfection, Expressing

Effects of Bulbophyllum drymoglossum aqueous extract (BDAE) on cell viability, proliferation, and chemical composition. (A) Representative images of Bulbophyllum drymoglossum leaves and aqueous extract (BDAE) preparation by high-temperature extraction (110°C, 15 min). (B) Relative viability of NIH/3T3 fibroblasts and porcine alveolar macrophages (3D4/31) after 24 h exposure to increasing concentrations of BDAE, measured using the WST-8 assay. ns, not significant; **** p<0.0001 versus vehicle control. (C) Effects of BDAE on cell proliferation in 3D4/31 cells during 3 days of culture with indicated extract concentrations. (D) Representative GC–MS total ion chromatogram of BDAE, showing major metabolite peaks. (E) Table of major compounds in BDAE as identified by GC-MS, including retention time, area percentage, compound names, similarity index (SI), and molecular formula. GC-MS, gas chromatography–mass spectrometry.

Journal: Animal Bioscience

Article Title: Bulbophyllum drymoglossum aqueous extract modulates immunometabolism and oxidative stress in porcine alveolar macrophages

doi: 10.5713/ab.25.0638

Figure Lengend Snippet: Effects of Bulbophyllum drymoglossum aqueous extract (BDAE) on cell viability, proliferation, and chemical composition. (A) Representative images of Bulbophyllum drymoglossum leaves and aqueous extract (BDAE) preparation by high-temperature extraction (110°C, 15 min). (B) Relative viability of NIH/3T3 fibroblasts and porcine alveolar macrophages (3D4/31) after 24 h exposure to increasing concentrations of BDAE, measured using the WST-8 assay. ns, not significant; **** p<0.0001 versus vehicle control. (C) Effects of BDAE on cell proliferation in 3D4/31 cells during 3 days of culture with indicated extract concentrations. (D) Representative GC–MS total ion chromatogram of BDAE, showing major metabolite peaks. (E) Table of major compounds in BDAE as identified by GC-MS, including retention time, area percentage, compound names, similarity index (SI), and molecular formula. GC-MS, gas chromatography–mass spectrometry.

Article Snippet: Porcine alveolar macrophages (3D4/31; ATCC CRL-2844) and mouse embryonic fibroblasts (NIH3T3; ATCC CRL-1658) were maintained at 37°C in a 5% CO 2 humidified incubator.

Techniques: Extraction, Control, Gas Chromatography-Mass Spectrometry, Gas Chromatography, Mass Spectrometry